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Bottlenecking problems of 2D cell culture technology
Advantages of DASEA bio-manufacturing platform
Subjective indicators, experience dependent
digitalization of whole process and consumables
labor intensive, low utilization rate of consumables
automatic and programable process control
insufficient use of space
3D culture, large single-batch production yield
open space operation
enclosed system, less contamination
Repeated enzyme digestion
better cell activity with biomimetic microcarriers
Expected cell harvest
Type of cultivation
Facility
Time
Labor
Reagent & consumables (thousand yuan)
Total cost (thousand yuan)
1*1010cells
2D
200㎡
1200h
10人
19.53
28.32
DASEA 3D
100㎡
120h
1人
9.60
13.30
90
%Facility
90
%Labour
80
%Time
52
%Reagents & consumables
62
%Total cost
Cell morphology / Viability / Cell count
Surface marker analysis of hUMSCs
Human STR profiling identification
Induced in vitro differentiation capacity test (Tri-lineage differentiation)
Chromosomal karyotyping
Telomerase activity assay
Soft agar colony formation assay
Nude mouse inoculation test
Bacterial/Fungal detection
Mycoplasma detection
Adventitious virus testing (Human / Animal-derived)
Endotoxin testing
Lysate residue
Microcarrier lysate residue testing
Cell secretory factors
Cellular senescence assay
Angiogenesis assay
Fibroblast colony-forming units (CFU-F)
Cell migration
Liver failure treatment
Intrauterine adhesion treatment
The statistical data as of Nov 4, 2024
Indications
Mechanism study
Production process
Quality inspection
Pre-clinical animal study
IIT
Intrauterine adhesions
Intervertebral disc degeneration
Refractory wounds
Acute liver failure
Chronic kidney disease
Tendon injur
0755 2641 2015
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